Anti-TNIP2 antibody [EPR17434] (ab205925)
![Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.com/ps/products/205/ab205925/Images/ab205925-250152-ab205925WBa.jpg "Anti-TNIP2 antibody [EPR17434] (ab205925)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17434] to TNIP2
- Suitable for: Flow Cyt, ICC/IF, IP, WB
- Reacts with: Human
Overview
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Product name
Anti-TNIP2 antibody [EPR17434]
See all TNIP2 primary antibodies -
Description
Rabbit monoclonal [EPR17434] to TNIP2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and HepG2 whole cell lysates; Human fetal liver lysate. ICC/IF: HepG2 and Jurkat cells. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17434 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TNIP2 antibody [EPR17434] (ab205925)All lanes : Anti-TNIP2 antibody [EPR17434] (ab205925) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.com/ps/products/205/ab205925/Images/ab205925-250151-ab205925WBb.jpg)
Western blot - Anti-TNIP2 antibody [EPR17434] (ab205925)Anti-TNIP2 antibody [EPR17434] (ab205925) at 1/1000 dilution + Human fetal liver lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.com/ps/products/205/ab205925/Images/ab205925-250150-ab205925IFa.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TNIP2 antibody [EPR17434] (ab205925)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling TNIP2 with ab205925 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear and cytoplasmic staining on HepG2 cell line.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab205925 at 1/50 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.com/ps/products/205/ab205925/Images/ab205925-250148-ab205925IFb.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TNIP2 antibody [EPR17434] (ab205925)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling TNIP2 with ab205925 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear and cytoplasmic staining on Jurkat cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab205925 at 1/50 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
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![Flow Cytometry - Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.com/ps/products/205/ab205925/Images/ab205925-262002-ab205925FC1.jpg)
Flow Cytometry - Anti-TNIP2 antibody [EPR17434] (ab205925)Flow Cytometry analysis of Jurkat cells labelling TNIP with ab203829 at 1/90 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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![Immunoprecipitation - Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.com/ps/products/205/ab205925/Images/ab205925-250146-ab205925IP.jpg)
Immunoprecipitation - Anti-TNIP2 antibody [EPR17434] (ab205925)TNIP2 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab205925 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab205925 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10ug (Input).
Lane 2: ab205925 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205925 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.com/ps/products/205/ab205925/Images/ab205925-8-benefits-of-recombinant-antibodies.png)
Anti-TNIP2 antibody [EPR17434] (ab205925)
