Anti-PHRF1 antibody (ab85974)
Key features and details
- Rabbit polyclonal to PHRF1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PHRF1 antibody
See all PHRF1 primary antibodies -
Description
Rabbit polyclonal to PHRF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residues 1400 and 1450 of Human PHRF1 (NP_065952.1).
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Positive control
- HeLa, 293T and NIH3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PHRF1 antibody (ab85974)All lanes : Anti-PHRF1 antibody (ab85974) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 179 kDa
Observed band size: 179 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-PHRF1 antibody (ab85974)Detection of PHRF1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85974 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.
