Anti-DBC-1 antibody (ab244377)
Key features and details
- Rabbit polyclonal to DBC-1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DBC-1 antibody
See all DBC-1 primary antibodies -
Description
Rabbit polyclonal to DBC-1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Orangutan
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Immunogen
Recombinant fragment corresponding to Human DBC-1 aa 550-650.
Database link: Q8N163 -
Positive control
- IHC-P: Human testis and kidney tissue. WB: RT4, NIH/3T3, NBT-II and U-251 MG cell lysate. Human liver and tonsil tissue lysate. ICC/IF: U-2 OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DBC-1 antibody (ab244377)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for DBC-1 (green) using ab244377 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody (ab244377)Paraffin-embedded human testis tissue stained for DBC-1 using ab244377 at 1/500 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody (ab244377)Paraffin-embedded human kidney tissue stained for DBC-1 using ab244377 at 1/500 dilution in immunohistochemical analysis. -
Western blot - Anti-DBC-1 antibody (ab244377)All lanes : Anti-DBC-1 antibody (ab244377) at 0.4 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (Rat cell line) cell lysate
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Western blot - Anti-DBC-1 antibody (ab244377)All lanes : Anti-DBC-1 antibody (ab244377) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody (ab244377)Paraffin-embedded human pancreas tissue stained for DBC-1 using ab244377 at 1/500 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody (ab244377)Paraffin-embedded human liver tissue stained for DBC-1 using ab244377 at 1/500 dilution in immunohistochemical analysis.
Low expression as expected.
