Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DBC-1 antibody [EPR18317] (ab205526)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18317] to DBC-1
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-DBC-1 antibody [EPR18317]
    See all DBC-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18317] to DBC-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and 293 whole cell lysates; Human fetal kidney lysate. IHC-P: Human cervical carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

     Previously labelled as KIAA1967. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DBC-1 antibody [EPR18317] (ab205526)
    Western blot - Anti-DBC-1 antibody [EPR18317] (ab205526)
    All lanes : Anti-DBC-1 antibody [EPR18317] (ab205526) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 103 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody [EPR18317] (ab205526)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody [EPR18317] (ab205526)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling DBC-1 with ab205526 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human cervical carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-DBC-1 antibody [EPR18317] (ab205526)
    Immunocytochemistry/ Immunofluorescence - Anti-DBC-1 antibody [EPR18317] (ab205526)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DBC-1 with ab205526 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab205526 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow Cytometry - Anti-DBC-1 antibody [EPR18317] (ab205526)
    Flow Cytometry - Anti-DBC-1 antibody [EPR18317] (ab205526)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DBC-1 with ab205526 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Anti-DBC-1 antibody [EPR18317] (ab205526)
    Anti-DBC-1 antibody [EPR18317] (ab205526)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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