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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Estrogen

Anti-DBC-1 antibody (ab70242)

Anti-DBC-1 antibody (ab70242)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to DBC-1
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-DBC-1 antibody
    See all DBC-1 primary antibodies
  • Description

    Rabbit polyclonal to DBC-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human DBC-1 aa 875-923 (C terminal).
    Database link: Q8N163

  • Positive control

    • Nuclear extract from HeLa cells
  • General notes

    Previously labelled as KIAA1967. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide

    Tris-citrate/phosphate buffer, pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Estrogen
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Estrogen
    • Epigenetics and Nuclear Signaling
    • Transcription
    • RNA polymerase
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Elongation factors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway

Images

  • Western blot - Anti-DBC-1 antibody (ab70242)
    Western blot - Anti-DBC-1 antibody (ab70242)
    Lanes 1-2 : Anti-DBC-1 antibody (ab70242) at 1/1000 dilution
    Lanes 3-4 : Anti-DBC-1 antibody (ab70242) at 1/10000 dilution

    Lanes 1 & 3 : Nuclear extract from HeLa cells at 15 µg
    Lanes 2 & 4 : Nuclear extract from HeLa cells at 45 µg

    Developed using the ECL technique.

    Predicted band size: 103 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody (ab70242)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody (ab70242)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling DBC-1 with ab70242 at 1/1000 (1µg/ml). Detection: DAB.

  • Immunoprecipitation - Anti-DBC-1 antibody (ab70242)
    Immunoprecipitation - Anti-DBC-1 antibody (ab70242)
    Immunoprecipitation: ab70242 used at 1 µg/100 µg of nuclear extract from HeLa cells. Western blot: 1/1000 ab70242. Detection: Chemiluminescence with exposure times of 5 mins

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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