Anti-DBC-1 antibody [EPR18317] - BSA and Azide free (ab251430)
![Anti-DBC-1 antibody [EPR18317] - BSA and Azide free (ab251430)](https://www.abcam.com/ps/products/251/ab251430/Images/ab251430-408270-anti-dbc-1-antibody-epr18317-bsa-and-azide-free-western-blot.jpg "Anti-DBC-1 antibody [EPR18317] - BSA and Azide free (ab251430)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18317] to DBC-1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-DBC-1 antibody [EPR18317] - BSA and Azide free
See all DBC-1 primary antibodies -
Description
Rabbit monoclonal [EPR18317] to DBC-1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251430 is the carrier-free version of ab205526.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18317 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DBC-1 antibody [EPR18317] - BSA and Azide free (ab251430)All lanes : Anti-DBC-1 antibody [EPR18317] (ab205526) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 103 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis data was developed using ab205526, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody [EPR18317] - BSA and Azide free (ab251430)](https://www.abcam.com/ps/products/251/ab251430/Images/ab251430-3-anti-dbc-1-antibody-epr18317-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody [EPR18317] - BSA and Azide free (ab251430)This data was developed using ab205526, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling DBC-1 with ab205526 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human cervical carcinoma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunocytochemistry - Anti-DBC-1 antibody [EPR18317] - BSA and Azide free (ab251430)](https://www.abcam.com/ps/products/251/ab251430/Images/ab251430-2-anti-dbc-1-antibody-epr18317-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-DBC-1 antibody [EPR18317] - BSA and Azide free (ab251430)This data was developed using ab205526, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DBC-1 with ab205526 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab205526 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
![Flow Cytometry - Anti-DBC-1 antibody [EPR18317] - BSA and Azide free (ab251430)](https://www.abcam.com/ps/products/251/ab251430/Images/ab251430-1-anti-dbc-1-antibody-epr18317-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-DBC-1 antibody [EPR18317] - BSA and Azide free (ab251430)This data was developed using ab205526, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DBC-1 with ab205526 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody. -
![Anti-DBC-1 antibody [EPR18317] - BSA and Azide free (ab251430)](https://www.abcam.com/ps/products/251/ab251430/Images/ab251430-5-benefits-of-recombinant-antibodies.png)
Anti-DBC-1 antibody [EPR18317] - BSA and Azide free (ab251430)