Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TAF3 antibody [EPR15598] (ab188332)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15598] to TAF3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-TAF3 antibody [EPR15598]
    See all TAF3 primary antibodies

  • Description

    Rabbit monoclonal [EPR15598] to TAF3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562, HeLa, HepG2 and 293 cell lysate. Human colon and Human papillary adenocarcinoma of thyroid tissue. HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15598

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF3 antibody [EPR15598] (ab188332)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF3 antibody [EPR15598] (ab188332)

    Immunohistochemical analysis of paraffin-embedded Human papillary adenocarcinoma of thyroid tissue, labeling TAF3 with ab188332 at 1/1000 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TAF3 antibody [EPR15598] (ab188332)
    Immunocytochemistry/ Immunofluorescence - Anti-TAF3 antibody [EPR15598] (ab188332)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling TAF3 with purified ab188332 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • Western blot - Anti-TAF3 antibody [EPR15598] (ab188332)
    Western blot - Anti-TAF3 antibody [EPR15598] (ab188332)
    All lanes : Anti-TAF3 antibody [EPR15598] (ab188332) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100 dilution

    Predicted band size: 104 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF3 antibody [EPR15598] (ab188332)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF3 antibody [EPR15598] (ab188332)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling TAF3 with ab188332 at 1/1000 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TAF3 antibody [EPR15598] (ab188332)
    Immunocytochemistry/ Immunofluorescence - Anti-TAF3 antibody [EPR15598] (ab188332)

    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling TAF3 (green) with ab188332 at 1/100 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

  • Anti-TAF3 antibody [EPR15598] (ab188332)
    Anti-TAF3 antibody [EPR15598] (ab188332)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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