Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15598] to TAF3 - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TAF3 antibody [EPR15598] - BSA and Azide free
    See all TAF3 primary antibodies

  • Description

    Rabbit monoclonal [EPR15598] to TAF3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562, HeLa, HepG2 and 293 cell lysate. Human colon and Human papillary adenocarcinoma of thyroid tissue. HeLa cells.

  • General notes

    ab215824 is the carrier-free version of ab188332.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15598

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)
    This data was developed using ab188332, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human papillary adenocarcinoma of thyroid tissue, labeling TAF3 with ab188332 at 1/1000 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)
    Immunocytochemistry - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)
    This data was developed using ab188332, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling TAF3 with purified ab188332 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue). Control: PBS only
  • Western blot - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)
    Western blot - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)
    All lanes : Anti-TAF3 antibody [EPR15598] (ab188332) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100 dilution

    Predicted band size: 104 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab188332, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)
    This data was developed using ab188332, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling TAF3 with ab188332 at 1/1000 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)
    Immunocytochemistry - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)
    This data was developed using ab188332, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling TAF3 (green) with ab188332 at 1/100 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
  • Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)
    Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)

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