Antibodies, Proteins, Kits and Reagents for Life Science

Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15598] to TAF3 - BSA and Azide free
Suitable for: IHC-P, ICC, WB
Reacts with: Human

Product name

Anti-TAF3 antibody [EPR15598] - BSA and Azide free
See all TAF3 primary antibodies
Description

Rabbit monoclonal [EPR15598] to TAF3 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, ICC, WBmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- K562, HeLa, HepG2 and 293 cell lysate. Human colon and Human papillary adenocarcinoma of thyroid tissue. HeLa cells.
General notes
ab215824 is the carrier-free version of ab188332.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15598
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)

This data was developed using ab188332, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human papillary adenocarcinoma of thyroid tissue, labeling TAF3 with ab188332 at 1/1000 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)

This data was developed using ab188332, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling TAF3 with purified ab188332 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue). Control: PBS only
Western blot - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)

All lanes : Anti-TAF3 antibody [EPR15598] (ab188332) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100 dilution

Predicted band size: 104 kDa
Observed band size: 140 kDa
why is the actual band size different from the predicted?

This data was developed using ab188332, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)

This data was developed using ab188332, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling TAF3 with ab188332 at 1/1000 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)

This data was developed using ab188332, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling TAF3 (green) with ab188332 at 1/100 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
Anti-TAF3 antibody [EPR15598] - BSA and Azide free (ab215824)

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