Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2581Y] to PIAS1 + PIAS2
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-PIAS1 + PIAS2 antibody [EPR2581Y]
    See all PIAS1+PIAS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2581Y] to PIAS1 + PIAS2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PIAS1 aa 600 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: O75925

  • Positive control

    • Daudi, 293, Jurkat, SW40 and HepG2 cell lysates; human tonsil tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)
    Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)
    All lanes : Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231) at 1/50000 dilution

    Lane 1 : Human PIAS1 full-length recombinant protein with GST-Tag
    Lane 2 : Human PIAS2-beta recombinant protein fragment with GST-His-Tag
    Lane 3 : Human PIAS3 recombinant protein fragment with SUMO-His-Tag

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 72 kDa
    Observed band size: 98, 42 kDa
    why is the actual band size different from the predicted?



    Blocking and Diluting buffer and concentration: 5% NFDM /TBST

    Exposure time: Lane1:10 seconds, Lane2: 5 seconds, Lane3: 3 minutes.

    Human PIAS1 full-length recombinant protein (Cat#: ab152888)

  • Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)
    Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)
    All lanes : Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231) at 1/50000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : SW40 cell lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 72 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)
    Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling PIAS1 + PIAS2 with purified ab77231 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)

    ab77231, at a 1/100 dilution, staining PIAS1 + PIAS2 in paraffin embedded human tonsil tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)
    Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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