Anti-Intron-encoded endonuclease I-SceI antibody (ab216263)
Key features and details
- Rabbit polyclonal to Intron-encoded endonuclease I-SceI
- Suitable for: WB, ICC/IF
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Intron-encoded endonuclease I-SceI antibody -
Description
Rabbit polyclonal to Intron-encoded endonuclease I-SceI -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant full length protein corresponding to Saccharomyces cerevisiae Intron-encoded endonuclease I-SceI aa 1 to the C-terminus. The immunogen was tagged with a 6xHis tag and an S tag.
Database link: P03882 -
Positive control
- U2OS cells transfected with GFP-SCE1; 293T cells transfected with GFP-SCE1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Intron-encoded endonuclease I-SceI antibody (ab216263)All lanes : Anti-Intron-encoded endonuclease I-SceI antibody (ab216263) at 1/2000 dilution
Lane 1 : Non-transfected 293T cells lysate
Lane 2 : 293T cells transfected with GFP-SCE1 lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 18 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Intron-encoded endonuclease I-SceI antibody (ab216263)Immunofluorescent analysis U2OS cells transfected with GFP-SCE1 (Green) labeling SCE1 using ab216263 at 1/600 dilution (red). DAPI staining is showed and the merge figure too