Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HtrA2 / Omi antibody [EPR22] - BSA and Azide free (ab247420)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22] to HtrA2 / Omi - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HtrA2 / Omi antibody [EPR22] - BSA and Azide free
    See all HtrA2 / Omi primary antibodies

  • Description

    Rabbit monoclonal [EPR22] to HtrA2 / Omi - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human HtrA2/ Omi. The exact sequence is proprietary.

  • General notes

    ab247420 is the carrier-free version of ab75982. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247420 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-HtrA2 / Omi antibody [EPR22] - BSA and Azide free (ab247420)
    Western blot - Anti-HtrA2 / Omi antibody [EPR22] - BSA and Azide free (ab247420)
    All lanes : Anti-HtrA2 / Omi antibody [EPR22] (ab75982) at 1/5000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 49 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab75982, the same antibody clone in a different buffer formulation.

    Secondary antibody - goat anti-rabbit HRP (ab6721).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [EPR22] - BSA and Azide free (ab247420)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [EPR22] - BSA and Azide free (ab247420)
    This data was developed using ab75982, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon using ab75982 at 1/100-1/250 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-HtrA2 / Omi antibody [EPR22] - BSA and Azide free (ab247420)
    Flow Cytometry - Anti-HtrA2 / Omi antibody [EPR22] - BSA and Azide free (ab247420)
    This data was developed using ab75982, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab75982 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75982, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-rabbit Alexa Fluor® 488 (IgG H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-HtrA2 / Omi antibody [EPR22] - BSA and Azide free (ab247420)
    Anti-HtrA2 / Omi antibody [EPR22] - BSA and Azide free (ab247420)

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