Human UCHL1 (PGP9.5) knockout HEK-293T cell line (ab255443)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Kidney -
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 11, 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 15, 20 TH01: 7, 9.3 TPOX: 11, 12 CSF1PO: 12 -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Research areas
Images
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Western blot - Human UCHL1 knockout HEK293T cell line (ab255443)All lanes : Anti-PGP9.5 antibody [EPR4118] (ab108986) at 1/1000 dilution
Lane 1 : Wild-type Hap1 cell lysate at 20 µg
Lane 2 : UCHL1 knockout Hap1 cell lysate at 20 µg
Lane 3 : SH-SY5Y cell lysate at 20 µg
Lane 4 : Wild-type HEK-293T cell lysate at 20 µg
Lane 5 : UCHL1 knockout HEK-293T cell lysate
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 24 kDa
Additional bands at: 37 kDa (possible Loading Control)Lanes 1 - 5: Merged signal (red and green). Green - ab108986 observed at 25 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab108986 was shown to react with PGP9.5 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255443 (knockout cell lysate ab263773) was used. Wild-type and PGP9.5 knockout samples were subjected to SDS-PAGE. ab108986 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human UCHL1 knockout HEK293T cell line (ab255443)Homozygous: 45 bp deletion in exon 1.