Human UCHL1 (PGP9.5) knockout HEK-293T cell lysate (ab263773)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab255547 - Human UCHL1 knockout HEK293T cell lysate 1 x 100µg ab255594 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 11, 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 15, 20 TH01: 7, 9.3 TPOX: 11, 12 CSF1PO: 12
Images
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Western blot - Human UCHL1 knockout HEK293T cell lysate (ab263773)
Lane 1: Wild-type Hap1 cell lysate (20 µg)
Lane 2: UCHL1 knockout Hap1 cell lysate (20 µg)
Lane 3: SH-SY5Y cell lysate (20 µg)
Lane 4: Wild-type HEK-293T cell lysate (20 µg)
Lane 5: UCHL1 knockout HEK-293T cell lysate (20 µg)
Lanes 1 - 5: Merged signal (red and green). Green - ab108986 observed at 25 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab108986 was shown to react with PGP9.5 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255443 (knockout cell lysate ab263773) was used. Wild-type and PGP9.5 knockout samples were subjected to SDS-PAGE. ab108986 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human UCHL1 knockout HEK293T cell lysate (ab263773)Homozygous: 45 bp deletion in exon 1