Human Angiopoietin 2 ELISA Kit (ANG2) (ab99971)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 4.12 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Angiopoietin 2 Microplate (12 x 8 well strip) 1 unit Biotinylated anti-Human Angiopoietin 2 (lyophilized) 2 vials Recombinant Angiopoietin 2 Standard Human (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AGPT 2
- Agpt2
- ANG 2
see all -
Database links
- Entrez Gene: 285 Human
- Omim: 601922 Human
- SwissProt: O15123 Human
- Unigene: 583870 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab99971.
