Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (ab226890)
Key features and details
- Rabbit polyclonal to PHD2 / prolyl hydroxylase - C-terminal
- Suitable for: ICC/IF, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PHD2 / prolyl hydroxylase antibody - C-terminal
See all PHD2 / prolyl hydroxylase primary antibodies -
Description
Rabbit polyclonal to PHD2 / prolyl hydroxylase - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PHD2/ prolyl hydroxylase (C terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: Q9GZT9 -
Positive control
- IHC-Fr: Adult mouse cerebellum tissue. WB: HeLa, untreated and treated with cobalt (II) chloride, whole cell extracts. ICC/IF: DIV9 rat E18 primary cortical neuron, U-87 MG cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (ab226890)
U-87 MG cells stained for PHD2 (green) using ab226890 at 1/500 dilution in ICC/IF.
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Immunocytochemistry/ Immunofluorescence - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (ab226890)IV9 rat E18 primary cortical neuron cells stained for PHD2 (green) using ab226890 at 1/500 dilution in ICC/IF.
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Immunohistochemistry (Frozen sections) - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (ab226890)Frozen-sectioned adult mouse cerebellum tissue stained for PHD2 / prolyl hydroxylase using ab226890 at 1/250 dilution in immunohistochemical analysis.
Red: NF-H, stained by NF-H antibody diluted at 1/500.
Blue: DAPI. -
Western blot - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (ab226890)All lanes : Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (ab226890) at 1/2000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 200 µM cobalt (II) chloride for 24 hours whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa10% SDS-PAGE gel.
