Anti-PHD2 / prolyl hydroxylase antibody [366G/76/3] (ab103432)
![Anti-PHD2 / prolyl hydroxylase antibody [366G/76/3] (ab103432)](https://www.abcam.com/ps/products/103/ab103432/Images/ab103432-215856-ab103432FC.jpg "Anti-PHD2 / prolyl hydroxylase antibody [366G/76/3] (ab103432)")
Key features and details
- Mouse monoclonal [366G/76/3] to PHD2 / prolyl hydroxylase
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PHD2 / prolyl hydroxylase antibody [366G/76/3]
See all PHD2 / prolyl hydroxylase primary antibodies -
Description
Mouse monoclonal [366G/76/3] to PHD2 / prolyl hydroxylase -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
MANDSGGPGGPSPSERDRQYCELCG
, corresponding to amino acids 1-24 of Human PHD2/ prolyl hydroxylase -
Positive control
- MCF7 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
366G/76/3 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-PHD2 / prolyl hydroxylase antibody [366G/76/3] (ab103432)
Overlay histogram showing SHSY-5Y cells stained with ab103432 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab103432, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
