Anti-TIFA antibody [EPR20925-114] (ab239352)
![Anti-TIFA antibody [EPR20925-114] (ab239352)](https://www.abcam.com/ps/products/239/ab239352/Images/ab239352-451618-anti-tifa-antibody-epr20925114-western-blot-whole.jpg "Anti-TIFA antibody [EPR20925-114] (ab239352)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20925-114] to TIFA
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-TIFA antibody [EPR20925-114]
See all TIFA primary antibodies -
Description
Rabbit monoclonal [EPR20925-114] to TIFA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T and MOLT-4 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20925-114 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TIFA antibody [EPR20925-114] (ab239352)All lanes : Anti-TIFA antibody [EPR20925-114] (ab239352) at 1/1000 dilution
Lane 1 : TIFA knockout HEK-293T whole cell lysate
Lane 2 : Wild-type HEK-293T whole cell lysate
Lane 3 : MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution
Predicted band size: 21 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Data was kindly provided by collaborator.
ab239352 Anti-TIFA antibody [EPR20925-114] was shown to react with TIFA in HEK-293T cells in Western blot. Loss of signal was observed when TIFA knockout sample was used. Wild-type and TIFA knockout samples were subjected to SDS-PAGE. ab239352 was incubated 1 hour at room temperature at 1/1000 dilution . Blots were developed with Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/5000 dilution for 1 hour at room temperature before imaging. Anti-beta Tubulin was used as loading control.
Exposure time: 2 minutes.
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![Anti-TIFA antibody [EPR20925-114] (ab239352)](https://www.abcam.com/ps/products/239/ab239352/Images/ab239352-453144-benefitsofrecombinantantibodies.png)
Anti-TIFA antibody [EPR20925-114] (ab239352)