Anti-DAZAP1 antibody [EPR14400-60] (ab184183)
![Anti-DAZAP1 antibody [EPR14400-60] (ab184183)](https://www.abcam.com/ps/products/184/ab184183/Images/ab184183-410209-ab257912-anti-dazap1-antibody-epr14400-60-western-blot-human-knockout-lysate.jpg "Anti-DAZAP1 antibody [EPR14400-60] (ab184183)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14400-60] to DAZAP1
- Suitable for: WB, IP
- Reacts with: Human
Overview
-
Product name
Anti-DAZAP1 antibody [EPR14400-60]
See all DAZAP1 primary antibodies -
Description
Rabbit monoclonal [EPR14400-60] to DAZAP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Jurkat, HeLa, HepG2 and 293T cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14400-60 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)All lanes : Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DAZAP1 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDaLanes 1-4: Merged signal (red and green). Green - ab184183 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.
ab184183 Anti-DAZAP1 antibody [EPR14400-60] was shown to specifically react with DAZAP1 in wild-type HEK293T cells. The band observed in knockout cell line ab266470 (knockout cell lysate ab257912) lane below 43 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and DAZAP1 knockout samples were subjected to SDS-PAGE. ab184183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)](https://www.abcam.com/ps/products/184/ab184183/Images/ab184183-410180-ab257911-anti-dazap1-antibody-epr14400-60-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)All lanes : Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DAZAP1 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDaLanes 1-4: Merged signal (red and green). Green - ab184183 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.
ab184183 Anti-DAZAP1 antibody [EPR14400-60] was shown to specifically react with DAZAP1 in wild-type HEK293T cells. The band observed in knockout cell line ab266469 (knockout cell lysate ab257911) lane below 43 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and DAZAP1 knockout samples were subjected to SDS-PAGE. ab184183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)](https://www.abcam.com/ps/products/184/ab184183/Images/ab184183-216370-anti-dazap1-antibody-epr14400-60-western-blot.jpg)
Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)All lanes : Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
-
![Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)](https://www.abcam.com/ps/products/184/ab184183/Images/ab184183-216374-anti-dazap1-antibody-epr14400-60-immunoprecipitation.jpg)
Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)Western blot analysis of DAZAP1 in Jurkat cell lysate immunoprecipitated using ab184183 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Jurkat lysate.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
-
![Anti-DAZAP1 antibody [EPR14400-60] (ab184183)](https://www.abcam.com/ps/products/184/ab184183/Images/ab184183-5-benefits-of-recombinant-antibodies.png)
Anti-DAZAP1 antibody [EPR14400-60] (ab184183)
