Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Wednesday March 10, 2021

Anti-DAZAP1 antibody [EPR14400-60] (ab184183)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14400-60] to DAZAP1
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-DAZAP1 antibody [EPR14400-60]
See all DAZAP1 primary antibodies
Description

Rabbit monoclonal [EPR14400-60] to DAZAP1
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat, HeLa, HepG2 and 293T cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14400-60
Isotype

IgG
Research areas

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)

All lanes : Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DAZAP1 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa

Lanes 1-4: Merged signal (red and green). Green - ab184183 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.

ab184183 Anti-DAZAP1 antibody [EPR14400-60] was shown to specifically react with DAZAP1 in wild-type HEK293T cells. The band observed in knockout cell line ab266470 (knockout cell lysate ab257912) lane below 43 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and DAZAP1 knockout samples were subjected to SDS-PAGE. ab184183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)

All lanes : Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DAZAP1 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa

Lanes 1-4: Merged signal (red and green). Green - ab184183 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.

ab184183 Anti-DAZAP1 antibody [EPR14400-60] was shown to specifically react with DAZAP1 in wild-type HEK293T cells. The band observed in knockout cell line ab266469 (knockout cell lysate ab257911) lane below 43 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and DAZAP1 knockout samples were subjected to SDS-PAGE. ab184183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)

All lanes : Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/10000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa
Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)

Western blot analysis of DAZAP1 in Jurkat cell lysate immunoprecipitated using ab184183 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Jurkat lysate.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Anti-DAZAP1 antibody [EPR14400-60] (ab184183)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com