Anti-DAZAP1 antibody (ab240603)
Key features and details
- Rabbit polyclonal to DAZAP1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DAZAP1 antibody
See all DAZAP1 primary antibodies -
Description
Rabbit polyclonal to DAZAP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide within Human DAZAP1 aa 75-125. The exact sequence is proprietary. (GeneID 26528).
Database link: Q96EP5 -
Positive control
- WB: HEK-293T, HeLa, Jurkat, TCMK-1 and NIH/3T3 cell lysates. IP: HEK-293T cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-DAZAP1 antibody (ab240603) at 0.4 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 10 seconds
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DAZAP1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab240603 at 6 μg/mL. For western blotting, ab240603 was used at 1 µg/ml.
Lane 1: ab240603 used for IP at 6 µg/mg per reaction.
Lane 2: Control IgG.Detection: Chemiluminescence with an exposure time of 30 seconds.