Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DAZAP1 antibody (ab106851)

Key features and details

  • Rabbit polyclonal to DAZAP1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-DAZAP1 antibody
    See all DAZAP1 primary antibodies

  • Description

    Rabbit polyclonal to DAZAP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Mouse DAZAP1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab125891)

  • Positive control

    • This antibody gave a positive signal in Human, Mouse and Rat Testis tissue lysates.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab106851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
All applications
Chicken
Zebrafish
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    RNA-binding protein, which may be required during spermatogenesis.

  • Tissue specificity

    Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.

  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DAZ associated protein 1 antibody
    • DAZ-associated protein 1 antibody
    • Dazap1 antibody
    • DAZP1_HUMAN antibody
    • Deleted in azoospermia associated protein 1 antibody
    • Deleted in azoospermia-associated protein 1 antibody
    see all

Images

  • Western blot - Anti-DAZAP1 antibody (ab106851)
    Western blot - Anti-DAZAP1 antibody (ab106851)
    All lanes : Anti-DAZAP1 antibody (ab106851) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Testis (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 46 kDa, 57 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute

References (0)

Publishing research using ab106851? Please let us know so that we can cite the reference in this datasheet.

ab106851 has not yet been referenced specifically in any publications.

Images

  • Western blot - Anti-DAZAP1 antibody (ab106851)
    Western blot - Anti-DAZAP1 antibody (ab106851)
    All lanes : Anti-DAZAP1 antibody (ab106851) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Testis (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 46 kDa, 57 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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