Anti-DAZAP1 antibody (ab106851)
Key features and details
- Rabbit polyclonal to DAZAP1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DAZAP1 antibody
See all DAZAP1 primary antibodies -
Description
Rabbit polyclonal to DAZAP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse DAZAP1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab125891) -
Positive control
- This antibody gave a positive signal in Human, Mouse and Rat Testis tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications ChickenZebrafishApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).Target
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Function
RNA-binding protein, which may be required during spermatogenesis. -
Tissue specificity
Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 26528 Human
- Entrez Gene: 70248 Mouse
- Entrez Gene: 362836 Rat
- Omim: 607430 Human
- SwissProt: Q96EP5 Human
- SwissProt: Q9JII5 Mouse
- Unigene: 222510 Human
- Unigene: 148693 Mouse
see all -
Alternative names
- DAZ associated protein 1 antibody
- DAZ-associated protein 1 antibody
- Dazap1 antibody
see all
Images
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Western blot - Anti-DAZAP1 antibody (ab106851)All lanes : Anti-DAZAP1 antibody (ab106851) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Testis (Mouse) Tissue Lysate
Lane 3 : Testis (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa, 57 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Datasheets and documents
References (0)
ab106851 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-DAZAP1 antibody (ab106851)All lanes : Anti-DAZAP1 antibody (ab106851) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Testis (Mouse) Tissue Lysate
Lane 3 : Testis (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa, 57 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
