Rat IL-10 ELISA Kit (ab214566)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.89 pg/ml
- Range: 31.3 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat IL-10 ELISA Kit
See all IL-10 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 5.6% Inter-assay Sample n Mean SD CV% Overall 3 3.4% -
Sample type
Cell culture supernatant, Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.89 pg/ml -
Range
31.3 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 93 84% - 109% Serum 94 93% - 94% Cit plasma 86 85% - 89% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat
Does not react with: Cow -
Product overview
Rat IL-10 ELISA Kit (ab214566) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-10 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-10 with 4.89 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin-10 is an anti-inflammatory cytokine involved in inhibition of a variety of other cytokines, including IFN-gamma, TNF, IL-2 and GM-CSF.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 10X Rat IL-10 Capture Antibody 1 x 600µl 10X Rat IL-10 Detector Antibody 1 x 600µl Rat IL-10 Lyophilized Recombinant Protein 2 vials Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
Database links
- Entrez Gene: 25325 Rat
- SwissProt: P29456 Rat
- Unigene: 9868 Rat
Images
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Other - Rat IL-10 ELISA Kit (ab214566)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Interpolated concentrations of native IL-10 in rat cell culture supernatant samples.The concentrations of IL-10 were measured in duplicates, interpolated from the IL-10 standard curves and corrected for sample dilution. Undiluted samples are as follows: rat PBMC stimulated with PHA (10 µg/mL, 48 hours) supernatant 50%, unstimulated PBMC supernatant 100%, and spleen stimulated with concanavalin A (5 µg/mL, 48 hours) supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10 concentration was determined to be 1,174 pg/mL in PBMC stimulated with PHA supernatant, 230 pg/mL in unstimulated PBMC supernatant and 789 pg/mL in spleen stimulated with concanavalin A supernatant.
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Rat IL-10 standard curve comparison dataStandard curve comparison between rat IL-10 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Example of rat IL-10 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of rat IL-10 standard curve in Sample Diluent 25BS.Background-subtracted data values (mean +/- SD) are graphed.
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Rat peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA.Conditioned media was harvested after 48 hours. IL-10 was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-10 were measured in duplicate, interpolated from the IL-1beta standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10 concentration was determined to be 1,068 pg/mL in PHA stimulated PBMC supernatant and 227 pg/mL in unstimulated PBMC supernatant.
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Sandwich ELISA - Rat IL-10 ELISA Kit (ab214566)To learn more about the advantages of recombinant antibodies see here.
