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Cancer Tumor immunology Cytokines Interleukins

Rat IL-10 Matched Antibody Pair Kit (ab218796)

Rat IL-10 Matched Antibody Pair Kit (ab218796)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Rat
  • Range: 31 pg/ml - 2000 pg/ml

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Overview

  • Product name

    Rat IL-10 Matched Antibody Pair Kit
    See all IL-10 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    2.7 pg/ml
  • Range

    31 pg/ml - 2000 pg/ml
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Rat IL-10 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant rat IL-10.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214566. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Rat IL-10 Capture Antibody 2 x 50µg 1 x 50µg
    Rat IL-10 Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Rat IL-10 Lyophilized Protein 2 vials 1 vial
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
  • Function

    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificity

    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similarities

    Belongs to the IL-10 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P22301 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CSIF
    • Cytokine synthesis inhibitory factor
    • GVHDS
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • MGC126450
    • MGC126451
    • T-cell growth inhibitory factor
    • TGIF
    see all
  • Database links

    • Entrez Gene: 25325 Rat
    • SwissProt: P29456 Rat
    • Unigene: 9868 Rat

    Images

    • Rat IL-10 standard curve
      Rat IL-10 standard curve

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Rat IL-10 Matched Antibody Pair Kit (ab218796)
      Sandwich ELISA - Rat IL-10 Matched Antibody Pair Kit (ab218796)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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