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Epigenetics and Nuclear Signaling Transcription Other factors

Anti-PER3 antibody [4B9D7] (ab201940)

Anti-PER3 antibody [4B9D7] (ab201940)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [4B9D7] to PER3
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-PER3 antibody [4B9D7]
    See all PER3 primary antibodies
  • Description

    Mouse monoclonal [4B9D7] to PER3
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human PER3 aa 700-1000. (Purified from E.coli).
    Database link: P56645

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human PER3 (aa723-954) recombinant protein; PER3 (aa723-954)-hIgGFc transfected HEK293 cell lysate; HeLa cells; Human ovarian cancer and rectum cancer tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    4B9D7
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators

Images

  • Western blot - Anti-PER3 antibody [4B9D7] (ab201940)
    Western blot - Anti-PER3 antibody [4B9D7] (ab201940)
    Anti-PER3 antibody [4B9D7] (ab201940) at 1/500 dilution + Human PER3 (aa723-954) recombinant protein

    Predicted band size: 132 kDa



    Expected MWt is 50.7 kDa.

  • Western blot - Anti-PER3 antibody [4B9D7] (ab201940)
    Western blot - Anti-PER3 antibody [4B9D7] (ab201940)
    All lanes : Anti-PER3 antibody [4B9D7] (ab201940) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : PER3 (aa723-954)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 132 kDa



    (Expected MW is 50.7 kDa)

  • Immunocytochemistry/ Immunofluorescence - Anti-PER3 antibody [4B9D7] (ab201940)
    Immunocytochemistry/ Immunofluorescence - Anti-PER3 antibody [4B9D7] (ab201940)

    Immunofluorescent analysis of HeLa cells labeling PER3 with ab201940 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Flow Cytometry - Anti-PER3 antibody [4B9D7] (ab201940)
    Flow Cytometry - Anti-PER3 antibody [4B9D7] (ab201940)

    Flow cytometric analysis of HeLa cells labeling PER3 with ab201940 at 1/200 dilution (green) compared to a negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PER3 antibody [4B9D7] (ab201940)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PER3 antibody [4B9D7] (ab201940)

    Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling PER3 with ab201940 at 1/200 dilution, with DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PER3 antibody [4B9D7] (ab201940)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PER3 antibody [4B9D7] (ab201940)

    Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling PER3 with ab201940 at 1/200 dilution, with DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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