Anti-PER3 antibody [4B9D7] (ab201940)
Key features and details
- Mouse monoclonal [4B9D7] to PER3
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-PER3 antibody [4B9D7]
See all PER3 primary antibodies -
Description
Mouse monoclonal [4B9D7] to PER3 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human PER3 aa 700-1000. (Purified from E.coli).
Database link: P56645 -
Positive control
- Human PER3 (aa723-954) recombinant protein; PER3 (aa723-954)-hIgGFc transfected HEK293 cell lysate; HeLa cells; Human ovarian cancer and rectum cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4B9D7 -
Isotype
IgG2b -
Research areas
Images
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Anti-PER3 antibody [4B9D7] (ab201940) at 1/500 dilution + Human PER3 (aa723-954) recombinant protein
Predicted band size: 132 kDaExpected MWt is 50.7 kDa.
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All lanes : Anti-PER3 antibody [4B9D7] (ab201940) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : PER3 (aa723-954)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 132 kDa(Expected MW is 50.7 kDa)
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Immunofluorescent analysis of HeLa cells labeling PER3 with ab201940 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Flow cytometric analysis of HeLa cells labeling PER3 with ab201940 at 1/200 dilution (green) compared to a negative control (red).
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Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling PER3 with ab201940 at 1/200 dilution, with DAB staining.
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Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling PER3 with ab201940 at 1/200 dilution, with DAB staining.