Anti-DAZAP1 antibody (ab240604)
Key features and details
- Rabbit polyclonal to DAZAP1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-DAZAP1 antibody
See all DAZAP1 primary antibodies -
Description
Rabbit polyclonal to DAZAP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human DAZAP1 aa 357-407. The exact sequence is proprietary. (GeneID 26528).
Database link: Q96EP5 -
Positive control
- WB: HEK-293T, HeLa, Jurkat, TCMK-1 and NIH/3T3 cell lysate. IP: HEK-293T cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunoprecipitation - Anti-DAZAP1 antibody (ab240604)
DAZAP1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab240604. For western blotting, ab240604 was used at 1 µg/ml.
Lane 1: ab240604 used for IP at 6 µg/mg lysate.
Lane 2: Control IgG.Detection: Chemiluminescence with an exposure time of 30 seconds.
-
Western blot - Anti-DAZAP1 antibody (ab240604)All lanes : Anti-DAZAP1 antibody (ab240604) at 0.4 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 10 seconds
