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Anti-XIAP antibody (ab227196)

Anti-XIAP antibody (ab227196)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to XIAP
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-XIAP antibody
    See all XIAP primary antibodies
  • Description

    Rabbit polyclonal to XIAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Pig
  • Immunogen

    Recombinant fragment within Human XIAP (internal sequence). The exact sequence is proprietary.
    Database link: P98170

  • Positive control

    • WB: HeLa whole cell extracts. ICC/IF: HeLa cells. IHC-P: A549 xenograft.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Survivin / IAPs
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Inhibitors
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • IAPs
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • IAPs
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-XIAP antibody (ab227196)
    Western blot - Anti-XIAP antibody (ab227196)
    All lanes : Anti-XIAP antibody (ab227196) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : XIAP Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 57 kDa

  • Western blot - Anti-XIAP antibody (ab227196)
    Western blot - Anti-XIAP antibody (ab227196)
    All lanes : Anti-XIAP antibody (ab227196) at 1/500 dilution

    Lane 1 : WT HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
    Lane 2 : XIAP knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 57 kDa



    7.5% SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-XIAP antibody (ab227196)
    Immunocytochemistry/ Immunofluorescence - Anti-XIAP antibody (ab227196)

    Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for XIAP (green) using ab227196 (1/100 dilution) in ICC/IF.

    Counterstain: Hoechst 33342 (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XIAP antibody (ab227196)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XIAP antibody (ab227196)

    Paraffin embedded A549 (human lung carcinoma cell line) xenograft stained for XIAP with ab227196 (1/100 dilution) in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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