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Human CIRBP (CIRP) knockout HEK293T cell pellet (ab278870)

Overview

  • Product name

    Human CIRBP (CIRP) knockout HEK293T cell pellet
    See all CIRP kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon2.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.

  • Domain

    Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression.

  • Post-translational
    modifications

    Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs.
    Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.

  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins.
  • Information by UniProt

  • Alternative names

    • A18 hnRNP
    • A18HNRNP
    • cirbp
    • CIRBP_HUMAN
    • CIRP
    • Cold inducible RNA binding protein
    • Cold-inducible RNA-binding protein
    • Glycine rich RNA binding protein
    • Glycine-rich RNA-binding protein CIRP
    • testicular tissue protein Li 39
    see all

Properties

Images

  • Western blot - Human CIRBP (CIRP) knockout HEK293T cell pellet (ab278870)
    Western blot - Human CIRBP (CIRP) knockout HEK293T cell pellet (ab278870)

    Lane 1: Wild-type HEK-293T cell lysate (20 µg)

    Lane 2: CIRBP knockout HEK-293T cell lysate (20 µg)

    Lanes 1-2: Merged signal (red and green). Green - ab191885 observed at 19 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab191885 Anti-CIRP antibody [EPR18783] was shown to specifically react with CIRP in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266187 (knockout cell lysate ab257252) was used. Wild-type and CIRP knockout samples were subjected to SDS-PAGE. ab191885 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human CIRBP (CIRP) knockout HEK293T cell pellet (ab278870)
    Sanger Sequencing - Human CIRBP (CIRP) knockout HEK293T cell pellet (ab278870)
    Homozygous: 7 bp deletion in exon2

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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