Anti-TORC2 antibody [EPR3384(2)] (ab109081)
![Anti-TORC2 antibody [EPR3384(2)] (ab109081)](https://www.abcam.com/ps/products/109/ab109081/Images/ab109081-214695-anti-torc2-antibody-epr33842-western-blot.jpg "Anti-TORC2 antibody [EPR3384(2)] (ab109081)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3384(2)] to TORC2
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-TORC2 antibody [EPR3384(2)]
See all TORC2 primary antibodies -
Description
Rabbit monoclonal [EPR3384(2)] to TORC2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, Raji, and A673 cell lysates; Human tonsil tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3384(2) -
Isotype
IgG -
Research areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
Images
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Western blot - Anti-TORC2 antibody [EPR3384(2)] (ab109081) This image is courtesy of an Abreview submitted by Javier TriñanesAnti-TORC2 antibody [EPR3384(2)] (ab109081) at 1/5000 dilution + Ins-1, rat insulinoma cell line, nuclear cell lysate at 1200000 cells
Secondary
HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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![Western blot - Anti-TORC2 antibody [EPR3384(2)] (ab109081)](https://www.abcam.com/ps/products/109/ab109081/Images/ab109081-121637-anti-torc2-antibody-epr33842-western-blot.jpg)
Western blot - Anti-TORC2 antibody [EPR3384(2)] (ab109081)All lanes : Anti-TORC2 antibody [EPR3384(2)] (ab109081) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Raji cell lysate
Lane 3 : A673 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 73 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC2 antibody [EPR3384(2)] (ab109081)](https://www.abcam.com/ps/products/109/ab109081/Images/ab109081-121636-anti-torc2-antibody-epr33842-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC2 antibody [EPR3384(2)] (ab109081)ab109081 at 1/100 dilution staining Torc2 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-TORC2 antibody [EPR3384(2)] (ab109081)](https://www.abcam.com/ps/products/109/ab109081/Images/ab109081-4-benefits-of-recombinant-antibodies.png)
Anti-TORC2 antibody [EPR3384(2)] (ab109081)
