Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µl 281 433 ₸

Order now and get it on Wednesday March 10, 2021

Anti-TORC2 antibody [EPR20219] (ab184239)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20219] to TORC2
Suitable for: WB
Reacts with: Mouse, Rat, Human, Chinese hamster

Product name

Anti-TORC2 antibody [EPR20219]
See all TORC2 primary antibodies
Description

Rabbit monoclonal [EPR20219] to TORC2
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

Chinese hamster

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A20, CTLL-2, YAC-1, Neuro-2a, C6, L6, A-673, Jurkat, Raji, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal heart, fetal kidney and fetal brain lysates; Mouse and rat brain, kidney and spleen lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Rat Human Chinese hamster

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20219
Isotype

IgG
Research areas

Western blot - Anti-TORC2 antibody [EPR20219] (ab184239)

All lanes : Anti-TORC2 antibody [EPR20219] (ab184239) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 73 kDa
Observed band size: 78 kDa
why is the actual band size different from the predicted?

Exposure times: Lanes 1, 2: 3 minutes; Lane 3: 15 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-TORC2 antibody [EPR20219] (ab184239)

All lanes : Anti-TORC2 antibody [EPR20219] (ab184239) at 1/1000 dilution

Lane 1 : A20 (mouse reticulum sarcoma cell line) whole cell lysate
Lane 2 : CTLL-2 (mouse T lymphocyte cell line) whole cell lysate
Lane 3 : YAC-1 (mouse Moloney murine leukemia virus induced lymphoma lymphoblast cell line) whole cell lysate
Lane 4 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 5 : C6 (rat glial tumor cell line) whole cell lysate
Lane 6 : L6 (rat skeletal muscle cell line) whole cell lysate
Lane 7 : A-673 (human muscle Ewing's Sarcoma cell line) whole cell lysate
Lane 8 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 9 : Raji (human Burkitt's lymphoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 73 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?

Exposure times: Lanes 1-3: 10 seconds; Lanes 4-9: 3 minutes.

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-TORC2 antibody [EPR20219] (ab184239)

All lanes : Anti-TORC2 antibody [EPR20219] (ab184239) at 1/1000 dilution

Lane 1 : Mouse brain lysate at 10 µg
Lane 2 : Mouse kidney lysate at 10 µg
Lane 3 : Mouse spleen lysate at 10 µg
Lane 4 : Rat brain lysate at 10 µg
Lane 5 : Rat kidney lysate at 10 µg
Lane 6 : Rat spleen lysate at 10 µg
Lane 7 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
Lane 9 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 73 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?

Exposure times: Lanes 1-6: 3 minutes; Lanes 7-9: 15 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-TORC2 antibody [EPR20219] (ab184239)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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