Anti-Mitotic Cells antibody [8B3G] (ab8956)
![Anti-Mitotic Cells antibody [8B3G] (ab8956)](https://www.abcam.com/ps/products/8/ab8956/Images/ab8596_1.gif "Anti-Mitotic Cells antibody [8B3G] (ab8956)")
Key features and details
- Mouse monoclonal [8B3G] to Mitotic Cells
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Human, Zebrafish
- Isotype: IgM
Overview
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Product name
Anti-Mitotic Cells antibody [8B3G] -
Description
Mouse monoclonal [8B3G] to Mitotic Cells -
Host species
Mouse -
Specificity
Strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) this antibody clearly distinguishes these M-phase cells from cell at other stages of the cell cycle. Dynamic information can be obtained by combining BrdU incorporation with antibody staining, which can distinguish and quantitate the four major fractions of the cell cycle. This antibody can be used for flow cytometric analyses and immunocytochemistry, it is not suitable for immunoblotting. -
Tested applications
Suitable for: Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Human, Zebrafish -
Immunogen
Total cell lysate of the human bladder carcinoma cell line T24.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
8B3G -
Myeloma
Sp2/0-Ag14 -
Isotype
IgM -
Research areas
Images
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Flow Cytometry - Anti-Mitotic Cells antibody [8B3G] (ab8956)Dual parameter flow cytometric analysis of human colon cancer HT29 cells stained with monoclonal antibody 8B3G and propidium iodide (PI). The mitotic cell fraction is encircled.
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![Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)](https://www.abcam.com/ps/products/8/ab8956/Images/Mitotic-Cells-Primary-antibodies-ab8956-1.jpg)
Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections). Samples ware frozen sections fixed in Acetone:Methanol 1:1. ab8956 used at 1:50 dilution and incubated for 45 minutes at room temperature. -
![Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)](https://www.abcam.com/ps/products/8/ab8956/Images/Mitotic-Cells-Primary-antibodies-ab8956-2.jpg)
Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections). Samples ware frozen sections fixed in Acetone:Methanol 1:1. ab8956 used at 1:50 dilution and incubated for 45 minutes at room temperature.