All lanes : Anti-Lipin 1 antibody [EPR3725] (ab92316) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HuT-78 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 99 kDa

ab92316 at a 1/50 dilution, staining Lipin 1 in paraffin-embedded Human colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ab92316 at a 1/50 dilution, staining Lipin 1 in paraffin-embedded Human uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunofluorescent staining of HepG2 cells using ab92316 at 1/100 dilution.

Overlay histogram showing HepG2 cells stained with ab92316 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92316, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.