Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Lipin 1 antibody [EPR3725] (ab92316)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3725] to Lipin 1
Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-Lipin 1 antibody [EPR3725]
See all Lipin 1 primary antibodies
Description

Rabbit monoclonal [EPR3725] to Lipin 1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Lipin 1 aa 150-250. The exact sequence is proprietary.
Database link: Q14693
Positive control
- Jurkat, HepG2 or HuT-78 cell lysate Human colon or uterus tissue
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3725
Isotype

IgG
Research areas

Western blot - Anti-Lipin 1 antibody [EPR3725] (ab92316)

All lanes : Anti-Lipin 1 antibody [EPR3725] (ab92316) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HuT-78 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 99 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] (ab92316)

ab92316 at a 1/50 dilution, staining Lipin 1 in paraffin-embedded Human colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] (ab92316)

ab92316 at a 1/50 dilution, staining Lipin 1 in paraffin-embedded Human uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Lipin 1 antibody [EPR3725] (ab92316)

Immunofluorescent staining of HepG2 cells using ab92316 at 1/100 dilution.
Flow Cytometry - Anti-Lipin 1 antibody [EPR3725] (ab92316)

Overlay histogram showing HepG2 cells stained with ab92316 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92316, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Anti-Lipin 1 antibody [EPR3725] (ab92316)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com