Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lipin 1 antibody (ab70138)

Key features and details

  • Rabbit polyclonal to Lipin 1
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

Overview

  • Product name

    Anti-Lipin 1 antibody
    See all Lipin 1 primary antibodies

  • Description

    Rabbit polyclonal to Lipin 1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Cow, Dog, Human, Pig, Chimpanzee

  • Immunogen

    Synthetic peptide corresponding to Human Lipin 1 aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab86631)

  • Positive control

    • This antibody gave a positive signal in the following Mouse Tissue Lysates: Liver; Pancreas, Kidney.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab70138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).

Target

  • Function

    Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.

  • Tissue specificity

    Abundant in adipose tissue and skeletal muscle. Lower levels in some portions of the digestive tract.

  • Involvement in disease

    Defects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM) [MIM:268200]; also known as acute recurrent rhabdomyolysis. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness and followed by excretion of myoglobin in the urine. Renal failure may occasionally occur. Onset is usually in early childhood under the age of 5 years.

  • Sequence similarities

    Belongs to the lipin family.

  • Domain

    Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.
    Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.

  • Post-translational
    modifications

    Phosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway (By similarity). Dephosphorylated in response to epinephrine and oleic acid.
    Sumoylated.

  • Cellular localization

    Nucleus. Cytoplasm > cytosol. Endoplasmic reticulum membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • EC=3.1.3.4 antibody
      • KIAA0188 antibody
      • Lipin-1 antibody
      • Lpin1 antibody
      • LPIN1_HUMAN antibody
      • PAP1 antibody
      • Phosphatidate phosphatase LPIN1 antibody
      see all

    Images

    • Western blot - Anti-Lipin 1 antibody (ab70138)
      Western blot - Anti-Lipin 1 antibody (ab70138)
      All lanes : Anti-Lipin 1 antibody (ab70138) at 1 µg/ml

      Lane 1 : Liver (Mouse) Tissue Lysate
      Lane 2 : Pancreas (Mouse) Tissue Lysate
      Lane 3 : Kidney (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 99 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?

    References (5)

    Publishing research using ab70138? Please let us know so that we can cite the reference in this datasheet.

    ab70138 has been referenced in 5 publications.

    • Guo Z  et al. mTORC2 Regulates Lipogenic Gene Expression through PPAR? to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. Biomed Res Int 2019:5196028 (2019). PubMed: 31223619
    • Lin YC  et al. Nifedipine Modulates Renal Lipogenesis via the AMPK-SREBP Transcriptional Pathway. Int J Mol Sci 20:N/A (2019). PubMed: 30934807
    • Lizardo K  et al. Diet Alters Serum Metabolomic Profiling in the Mouse Model of Chronic Chagas Cardiomyopathy. Dis Markers 2019:4956016 (2019). PubMed: 31949545
    • Zhang W  et al. Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury. Sci Rep 8:408 (2018). WB ; Mouse . PubMed: 29323242
    • Cai K  et al. Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells. Biochim Biophys Acta 1841:552-62 (2014). WB . PubMed: 24369117

    Images

    • Western blot - Anti-Lipin 1 antibody (ab70138)
      Western blot - Anti-Lipin 1 antibody (ab70138)
      All lanes : Anti-Lipin 1 antibody (ab70138) at 1 µg/ml

      Lane 1 : Liver (Mouse) Tissue Lysate
      Lane 2 : Pancreas (Mouse) Tissue Lysate
      Lane 3 : Kidney (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 99 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Alternative products to Anti-Lipin 1 antibody (ab70138)

    Sign up