Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171)
Key features and details
- Assay type: Quantitative
Overview
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Product name
Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer)
See all Mitochondria Isolation kits -
Assay type
Quantitative -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
ab110171 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 kit Dounce Homogenizer (2 ml size) with pestles 1 unit Reagent A 1 x 50ml Reagent B 1 x 50ml Reagent C 1 x 10ml -
Research areas
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Alternative names
- mitochondria purification
- mitochondrial isolation
- mitochondrial purification
Images
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Western blot - Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171)Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110171/MS853 Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.
