Mitochondria Isolation Kit for Cultured Cells (ab110170)
Key features and details
- Assay type: Quantitative
- Assay time: 1 hr
Overview
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Product name
Mitochondria Isolation Kit for Cultured Cells
See all Mitochondria Isolation kits -
Assay type
Quantitative -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Available with a dounce homogenizer as kit ab110171.
Mitochondrial isolation protocol summary:
- suspend cells in reagent A and incubate for 10 min
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant, repeat
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in reagent C and analyze or freeze
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 kit Reagent A 1 x 50ml Reagent B 1 x 50ml Reagent C 1 x 10ml -
Research areas
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Alternative names
- mitochondria purification
- mitochondrial isolation
- mitochondrial purification
Images
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Western blot - Mitochondria Isolation Kit for Cultured Cells (ab110170)Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110170 (MS852) Mitochondria Isolation Kit for Cultured Cells were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.
