Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1465Y] to Serine/threonine-protein kinase 4/MST-1 - BSA and Azide free
  • Suitable for: Flow Cyt, IP, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free

  • Description

    Rabbit monoclonal [EP1465Y] to Serine/threonine-protein kinase 4/MST-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human gastric carcinoma tissue.

  • General notes

    ab232551 is the carrier-free version of ab51134. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232551 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP1465Y

  • Isotype

    IgG

Images

  • Flow Cytometry - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)
    Flow Cytometry - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)

    Flow Cytometry analysis of HeLa cells labelling Serine/theronine-protein kinase 4 /MST-1 with purified ab51134 at a dilution of 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51134).

  • Immunoprecipitation - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)
    Immunoprecipitation - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)

    ab51134 immunoprecipitating Serine/threonine-protein kinase 4. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/30 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.

    Lane 1: Jurkat (human acute T cell leukemia) whole cell lysate (10ug)
    Lane 2: Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab51134 in Jurkat (human acute T cell leukemia) whole cell lysate

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51134).

  • Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)
    Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)

    ab51134 staining Serine/threonine-protein kinase 4 / MST-1 in Raw264.7 (mouse abelson murine leukemia virus-induced tumor) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a concentration of 1/1000. ab7291 anti-Tubulin (mouse mAb) (1/1000) and ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000)  were used as counterstains for primary antibody ab51134 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51134).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)

    ab51134 staining Serine/threonine-protein kinase 4 in human gastric carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51134).

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