Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1465Y] to Serine/threonine-protein kinase 4/MST-1 - BSA and Azide free
Suitable for: Flow Cyt, IP, IHC-P, WB, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free
Description

Rabbit monoclonal [EP1465Y] to Serine/threonine-protein kinase 4/MST-1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, IP, IHC-P, WB, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human gastric carcinoma tissue.
General notes
ab232551 is the carrier-free version of ab51134. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232551 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1465Y
Isotype

IgG

Flow Cytometry - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)

Flow Cytometry analysis of HeLa cells labelling Serine/theronine-protein kinase 4 /MST-1 with purified ab51134 at a dilution of 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour^® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51134).
Immunoprecipitation - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)

ab51134 immunoprecipitating Serine/threonine-protein kinase 4. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/30 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.

Lane 1: Jurkat (human acute T cell leukemia) whole cell lysate (10ug)
Lane 2: Jurkat (human acute T cell leukemia) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab51134 in Jurkat (human acute T cell leukemia) whole cell lysate

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51134).
Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)

ab51134 staining Serine/threonine-protein kinase 4 / MST-1 in Raw264.7 (mouse abelson murine leukemia virus-induced tumor) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a concentration of 1/1000. ab7291 anti-Tubulin (mouse mAb) (1/1000) and ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000) were used as counterstains for primary antibody ab51134 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51134).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4 antibody [EP1465Y] - BSA and Azide free (ab232551)

ab51134 staining Serine/threonine-protein kinase 4 in human gastric carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1: PBS in place of primary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51134).

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