Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer) (ab110169)
Key features and details
- Assay type: Quantitative
- Sample type: Tissue
Overview
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Product name
Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer)
See all Mitochondria Isolation kits -
Sample type
Tissue -
Assay type
Quantitative -
Product overview
ab110169 (MS851) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
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Notes
Tested tissues: brain, liver and heart
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 kit Dounce Homogenizer (2 ml size) with pestles 1 unit Isolation Buffer 1 x 100ml Wash Buffer 1 x 30ml -
Research areas
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Alternative names
- mitochondria purification
- mitochondrial isolation
- mitochondrial purification
Images
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Western blot - Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer) (ab110169)
Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 µg (lane 1) and 10 µg (lane 2) of mitochondria were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4
