Mitochondria Isolation Kit for Tissue (ab110168)
Key features and details
- Assay type: Quantitative
- Assay time: 1 hr
- Sample type: Tissue
Overview
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Product name
Mitochondria Isolation Kit for Tissue
See all Mitochondria Isolation kits -
Sample type
Tissue -
Assay type
Quantitative -
Assay time
1h 00m -
Product overview
ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Mitochondrial isolation protocol summary:
- suspend minced tissue in isolation buffer
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in isolation buffer, spin at 12,000g for 15 min, collect pellet and repeat spin
- resuspend pellet in isolation buffer and analyze or freeze -
Notes
Tested tissues: brain, liver and heart
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 kit Isolation Buffer 1 x 100ml Wash Buffer 1 x 30ml -
Research areas
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Alternative names
- mitochondria purification
- mitochondrial isolation
- mitochondrial purification
Images
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Western blot - Mitochondria Isolation Kit for Tissue (ab110168)
Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 µg (lane 1) and 10 µg (lane 2) of mitochondria were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4.
