Anti-DDX5 antibody (ab245471)
Key features and details
- Rabbit polyclonal to DDX5
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDX5 antibody
See all DDX5 primary antibodies -
Description
Rabbit polyclonal to DDX5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human DDX5 aa 550-600. The exact sequence is proprietary. NP_004387.1
Database link: P17844 -
Positive control
- WB: HeLa, HEK-293T, MCF7 and K562 whole cell lysate. IP: HeLa cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245471 was affinity purified using an epitope specific to DDX5 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-DDX5 antibody (ab245471)
DDX5 was immunoprecipitated from 1 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab245471 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245471 at 0.02 µg/ml.
Lane 1: ab245471 IP in HeLa cell lysate.
Lane 2: Control IgG in HeLa cell lysate.Detection: Chemiluminescence for an exposure time of 30 seconds.
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Western blot - Anti-DDX5 antibody (ab245471)All lanes : Anti-DDX5 antibody (ab245471) at 0.02 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 2 : HeLa whole cell lysate at 50 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 50 µg
Lane 5 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 30 seconds
