Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human ARMET/ARP protein (ab201386)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE, HPLC, Functional Studies

Description

  • Product name

    Recombinant human ARMET/ARP protein
    See all ARMET/ARP proteins and peptides

  • Biological activity

    Fully biologically active when compared to standard.

    The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg.

  • Purity

    > 95 % SDS-PAGE.
    > 95 % by HPLC.

  • Expression system

    Escherichia coli

  • Accession

    P55145

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL

    • Predicted molecular weight

      18 kDa

    • Amino acids

      25 to 182

    • Additional sequence information

      A single non-glycosylated polypeptide chain containing 158 amino acids. This product is the mature full length protein without the signal peptide.

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 100% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Briefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at

    General Info

    • Alternative names

      • Arginine rich mutated in early stage tumors
      • Arginine rich protein
      • Arginine-rich protein
      • ARMET
      • ARMET protein
      • ARP
      • Manf
      • MANF_HUMAN
      • Mesencephalic astrocyte derived neurotrophic factor
      • Mesencephalic astrocyte-derived neurotrophic factor
      • MGC142148
      • MGC142150
      • Protein ARMET
      see all

    • Function

      Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death.

    • Sequence similarities

      Belongs to the ARMET family.

    • Domain

      The N-terminal region may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response.

    • Post-translational
      modifications

      May contain sialic acid residues.

    • Cellular localization

      Secreted.
    • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

References (0)

Publishing research using ab201386? Please let us know so that we can cite the reference in this datasheet.

ab201386 has not yet been referenced specifically in any publications.

Images

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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