Anti-IRAK2 antibody (ab62419)
Key features and details
- Rabbit polyclonal to IRAK2
- Suitable for: ICC/IF, ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IRAK2 antibody
See all IRAK2 primary antibodies -
Description
Rabbit polyclonal to IRAK2 -
Host species
Rabbit -
Specificity
This antibody reacts with IRAK2, it does not react with IRAK -
Tested applications
Suitable for: ICC/IF, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A synthetic peptide corresponding to 17 amino acids near the C terminus of mouse IRAK2a, (which is common to all four isoforms).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-IRAK antibody (ab62419) reactivity with reduced wildtype (WT) or IRAK2 knockout (KO) mouse embryonic fibroblast (MEF) cell lysate. After SDS-PAGE, membranes were blocked in 5% milk for 1h at 25°C before incubation with ab62419 (1µg/ml dilution in 5% milk in TBS/0.5% Tween) for 16h at 4ºC. The blot was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.
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Immunofluorescent analysis of IRAK2 expression in human spleen cells with ab62419 at 20µg/ml.
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Lane 1 : Anti-IRAK2 antibody (ab62419) at 0.5 µg/ml
Lane 2 : Anti-IRAK2 antibody (ab62419) at 1 µg/ml
Lane 3 : Anti-IRAK2 antibody (ab62419) at 2 µg/ml
All lanes : RAW264.7 whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
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Immunocytochemistry of IRAK2 in A-20 cells with ab62419 antibody at 1 µg/ml.
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Immunofluorescence of IRAK2 in A20 cells using ab62419 at 10 ug/ml.