Anti-IRAK2 antibody [EPR16530] (ab192249)
![Anti-IRAK2 antibody [EPR16530] (ab192249)](https://www.abcam.com/ps/products/192/ab192249/Images/ab192249-229930-anti-irak2-antibody-epr16530-western-blot.jpg "Anti-IRAK2 antibody [EPR16530] (ab192249)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16530] to IRAK2
- Suitable for: Flow Cyt, IP, WB
- Reacts with: Human
Overview
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Product name
Anti-IRAK2 antibody [EPR16530]
See all IRAK2 primary antibodies -
Description
Rabbit monoclonal [EPR16530] to IRAK2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562, MOLT4, THP1 and HeLa cell lysates; MOLT4 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16530 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IRAK2 antibody [EPR16530] (ab192249)All lanes : Anti-IRAK2 antibody [EPR16530] (ab192249) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : MOLT4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 69 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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![Flow Cytometry - Anti-IRAK2 antibody [EPR16530] (ab192249)](https://www.abcam.com/ps/products/192/ab192249/Images/ab192249-229928-anti-irak2-antibody-epr16530-flow-cytometry.jpg)
Flow Cytometry - Anti-IRAK2 antibody [EPR16530] (ab192249)Flow cytometric analysis of 2% paraformaldehyde-fixed MOLT4 cells labeling IRAK2 with ab192249 at 1/220 dilution (pink), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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![Western blot - Anti-IRAK2 antibody [EPR16530] (ab192249)](https://www.abcam.com/ps/products/192/ab192249/Images/ab192249-229929-anti-irak2-antibody-epr16530-western-blot.jpg)
Western blot - Anti-IRAK2 antibody [EPR16530] (ab192249)All lanes : Anti-IRAK2 antibody [EPR16530] (ab192249) at 1/1000 dilution
Lane 1 : THP1 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 69 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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![Immunoprecipitation - Anti-IRAK2 antibody [EPR16530] (ab192249)](https://www.abcam.com/ps/products/192/ab192249/Images/ab192249-229927-anti-irak2-antibody-epr16530-immunoprecipitation.jpg)
Immunoprecipitation - Anti-IRAK2 antibody [EPR16530] (ab192249)Western blot analysis of IRAK2 in MOLT4 cell lysates immunoprecipitated using ab192249 at 1/60 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM /TBST.
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![Anti-IRAK2 antibody [EPR16530] (ab192249)](https://www.abcam.com/ps/products/192/ab192249/Images/ab192249-5-benefits-of-recombinant-antibodies.png)
Anti-IRAK2 antibody [EPR16530] (ab192249)
