Anti-Glypican 3 antibody (ab101478)
Key features and details
- Rabbit polyclonal to Glypican 3
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Glypican 3 antibody
See all Glypican 3 primary antibodies -
Description
Rabbit polyclonal to Glypican 3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human Glypican 3 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab113760) -
Positive control
- This antibody gave a positive signal in the following lysates: Human Kidney Tissue; Human Liver Tissue; HepG2 Whole Cell; HEK293 Whole Cell.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications MouseRatCowChimpanzeeApplication Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa.Notes ICC/IF
Use a concentration of 5 µg/ml.WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa.Target
-
Function
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. -
Tissue specificity
Highly expressed in lung, liver and kidney. -
Involvement in disease
Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. -
Sequence similarities
Belongs to the glypican family. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
-
Database links
- Entrez Gene: 465866 Chimpanzee
- Entrez Gene: 2719 Human
- Entrez Gene: 14734 Mouse
- Entrez Gene: 25236 Rat
- Omim: 300037 Human
- SwissProt: A5A6P7 Chimpanzee
- SwissProt: P51654 Human
- SwissProt: Q8CFZ4 Mouse
see all -
Alternative names
- DGSX antibody
- Glypican proteoglycan 3 antibody
- Glypican-3 [Precursor] antibody
see all
Images
-
Western blot - Anti-Glypican 3 antibody (ab101478)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Glypican 3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab101478 observed at 66 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab101478 was shown to specifically react with Glypican 3 in wild-type HAP1 cells as signal was lost in Glypican 3 knockout cells. Wild-type and Glypican 3 knockout samples were subjected to SDS-PAGE. ab101478 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Anti-Glypican 3 antibody (ab101478)All lanes : Anti-Glypican 3 antibody (ab101478) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 20 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Glypican 3 antibody (ab101478)ICC/IF image of ab101478 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (0)
ab101478 has not yet been referenced specifically in any publications.
Images
-
Western blot - Anti-Glypican 3 antibody (ab101478)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Glypican 3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab101478 observed at 66 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab101478 was shown to specifically react with Glypican 3 in wild-type HAP1 cells as signal was lost in Glypican 3 knockout cells. Wild-type and Glypican 3 knockout samples were subjected to SDS-PAGE. ab101478 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Anti-Glypican 3 antibody (ab101478)All lanes : Anti-Glypican 3 antibody (ab101478) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 20 minutes
-
Immunocytochemistry/ Immunofluorescence - Anti-Glypican 3 antibody (ab101478)ICC/IF image of ab101478 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
