Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20569] to Glypican 3 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free
    See all Glypican 3 primary antibodies

  • Description

    Rabbit monoclonal [EPR20569] to Glypican 3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human placenta and hepatocellular cancer tissues. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells. IP: HepG2 whole cell lysate.

  • General notes

    Ab240995 is the carrier-free version of ab207080. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240995 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Glypican 3 with ab207080 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human placenta (PMID: 12788060; PMID: 11193214). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207080)

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)
    Immunocytochemistry/ Immunofluorescence - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)

    Immunofluorescent analysis of 100% methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Glypican 3 with ab207080  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on HepG2 cell line. 

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Negative control: Raji (PMID: 9371521).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207080)
  • Flow Cytometry - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)
    Flow Cytometry - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)

    Left panel - Negative control: Flow cytometric analysis of 4% paraformaldehyde-fixed Raji (human Burkitt's lymphoma) cell line labeling Glypican 3 with ab207080 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    Right panel: Flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling Glypican 3 with ab207080 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207080)
  • Immunoprecipitation - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)
    Immunoprecipitation - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)

    This data was developed using ab207080, the same antibody clone in a different buffer formulation.
    Purified ab207080 at 1/40 dilution (2µg) immunoprecipitating Glypican 3 in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 µg
    Lane 2 (+): ab207080 + HepG2 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab207080 in HepG2 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/10,000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 40,70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer tissue labeling Glypican 3 with ab207080 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human hepatocellular cancer (PMID: 12788060). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207080)

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Glypican 3 with ab207080 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Negative control: No staining on human liver (PMID: 12788060).

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207080)

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)
    Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)

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