Anti-Glypican 3 antibody [EPR10641] (ab174851)
![Anti-Glypican 3 antibody [EPR10641] (ab174851)](https://www.abcam.com/ps/products/174/ab174851/Images/ab174851-298738-anti-glypican-3-antibody-epr10641-western-blot.jpg "Anti-Glypican 3 antibody [EPR10641] (ab174851)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10641] to Glypican 3
- Suitable for: Flow Cyt, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Glypican 3 antibody [EPR10641]
See all Glypican 3 primary antibodies -
Description
Rabbit monoclonal [EPR10641] to Glypican 3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Recombinant fragment within Human Glypican 3. The exact sequence is proprietary.
Database link: P51654 -
Positive control
- HepG2 whole cell lysate (ab7900); Human fetal liver lysate; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10641 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: GPC3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab174851 observed at 70 kDa. Red - loading control, ab130007, observed at 125 kDa.
ab174851 was shown to recognize Glypican 3 in wild-type HAP1 cells as signal was lost at the expected MW in GPC3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GPC3 knockout samples were subjected to SDS-PAGE. Ab174851 and ab130007 (Mouse anti-vinculin loading control) were incubated overnight at 4°C at 1/1000 and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-Glypican 3 antibody [EPR10641] (ab174851)](https://www.abcam.com/ps/products/174/ab174851/Images/ab174851-202329-anti-glypican-3-antibody-epr10641-flow-cytometry.jpg)
Flow Cytometry - Anti-Glypican 3 antibody [EPR10641] (ab174851)Flow cytometric analysis of permeabilized HepG2 cells labeling Glypican 3 using ab174851 at 1/10 dilution (red) or a rabbit IgG negative (green).
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![Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851)](https://www.abcam.com/ps/products/174/ab174851/Images/ab174851-202328-anti-glypican-3-antibody-epr10641-western-blot.jpg)
Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851)All lanes : Anti-Glypican 3 antibody [EPR10641] (ab174851) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 66 kDa
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![Anti-Glypican 3 antibody [EPR10641] (ab174851)](https://www.abcam.com/ps/products/174/ab174851/Images/ab174851-4-benefits-of-recombinant-antibodies.png)
Anti-Glypican 3 antibody [EPR10641] (ab174851)
