Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18113] to Brachyury / Bry - BSA and Azide free
  • Suitable for: IHC - Wholemount, IP, ICC/IF, IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free
    See all Brachyury / Bry primary antibodies

  • Description

    Rabbit monoclonal [EPR18113] to Brachyury / Bry - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse E14.5 embryo tissue. ICC: MUG-Chor1 (human sacral bone chordoma) cells.

  • General notes

    Ab236023 is the carrier-free version of ab209665. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236023 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)
    Immunocytochemistry/ Immunofluorescence - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)

    Ab209665 staining Brachyury/Bry in MUG-Chor1 (human sacral bone chordoma) cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Samples were incubated with primary antibody at a 1/1000 dilution.  An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at a 1/1000 dilution.  An Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as a counterstain antibody.  DAPI was used as nuclear counterstain. Nuclear staining on MUG-Chor1 cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

  • Immunoprecipitation - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)
    Immunoprecipitation - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)

    Lane 1 (input):  MUG-Chor1 (human sacral bone chordoma) whole cell lysate, 10 μg
    Lane 2 (+): MUG-Chor1 whole cell lysate
    Lane 3 (-):  Rabbit monoclonal IgG (ab172730) instead of  ab209665 in MUG-Chor1 whole cell lysate

    Ab209665 immunoprecipitating Brachyury/Bry in MUG-Chor1 lysates. For western blotting, primary antibody used was ab209665 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking and diluting buffer used was 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

  • Flow Cytometry - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)
    Flow Cytometry - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)

    Ab209665 staining Brachyury/Bry in MUG-Chor1 (Human sacral bone chordoma) cell line by Flow cytometry. Cells were fixed with 4% paraformaldehyde and 90% methanol. Sample was incubated with primary antibody at 1/400 dilution (red). A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution.  Rabbit monoclonal IgG (ab172730) (Black) was used as an isotype control. Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)

    Ab209665 staining Brachyury/Bry in Rat E14.5 embryo tissue sections by Immunohistochemistry (IHC-P). Tissue was fixed paraffin and antigen retrieval was performed by heat mediation using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at a 1/4000 dilution. A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counter-stain. Nuclear staining on notochord of rat E14.5 embryo.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

  • IHC - Wholemount - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)
    IHC - Wholemount - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023) This image is courtesy of an Abreview by Dr Sophie Morgani.

    IHC - Wholemount of E6.5 wholemount mouse embryo labelling Brachyury / Bry with ab209665. Sample was incubated with primary antibody (1/100 in PBS/0.1% Triton-X/5% donkey serum/1% BSA) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG monoclonal was used as the secondary antibody (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)

    Immunohistochemical analysis of paraffin-embedded Human chordoma tissue labeling Brachyury / Bry with ab209665 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human chordoma is observed. Counter stained with Hematoxylin

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Brachyury / Bry with ab209665 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human kidney. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)

    Immunohistochemical analysis of paraffin-embedded Human meningioma tissue labeling Brachyury / Bry with ab209665 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human meningioma. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)

    Immunohistochemical analysis of paraffin-embedded Human chondrosarcoma tissue labeling Brachyury / Bry with ab209665 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human chondrosarcoma. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)

    ab209665 staining Brachyury/Bry in mouse E14.5 embryo tissue sections by Immunohistochemistry (IHC-P). Tissue was fixed paraffin and antigen retrieval was performed by heat mediation using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at a 1/4000 dilution. A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counter-stain. Nuclear staining on notochord of mouse E14.5 embryo.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209665).

  • Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)
    Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)

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