Anti-SPANX antibody (ab51493)
Key features and details
- Rabbit polyclonal to SPANX
- Suitable for: IHC-P, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-SPANX antibody
See all SPANX primary antibodies -
Description
Rabbit polyclonal to SPANX -
Host species
Rabbit -
Specificity
Reacts with SPANX-C, SPANX-B and SPANX-N family members. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Recombinant fragment
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Immunogen
Full-length recombinant human SPANX-C protein.
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Positive control
- Nuclear extract from VWM105 cells, purified recombinant SPANX-B, SPANX-C and SPANX-N proteins.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab51493 was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SPANX antibody (ab51493)All lanes : Anti-SPANX antibody (ab51493) at 1/1000 dilution
Lane 1 : VWM105 cell nuclear extract
Lane 2 : purified recombinant SPANX-C fusion protein
Lane 3 : purified recombinant SPANX-B
Lane 4 : purified recombinant SPANX-C
Lane 5 : purified recombinant SPANX-N
Secondary
Lane 1 : IRDye™800 conjugated Goat anti Rabbit
IgG [H&L]
Lane 2 : IRDye™800 conjugated Goat anti Rabbit
IgG [H&L]
Predicted band size: 11 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPANX antibody (ab51493)ab51493 at 2.5 ug/ml staining human sperm and spermatids at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
