Anti-SPANX antibody (ab47252)
Key features and details
- Rabbit polyclonal to SPANX
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-SPANX antibody
See all SPANX primary antibodies -
Description
Rabbit polyclonal to SPANX -
Host species
Rabbit -
Specificity
The immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide:
GDSDPQPAPKKMKTSES
, corresponding to internal sequence amino acids 31-47 of Human SPANX C -
Positive control
- Nuclear extract from VWM105 cells
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General notes
Dilute only prior to immediate use.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SPANX antibody (ab47252)All lanes : Anti-SPANX antibody (ab47252) at 1/1000 dilution
Lane 1 : Nuclear extract from VWM105 cells
Lane 2 : purified recombinant
SPANX-C fusion protein.
Lane 3 : purified
recombinant SPANX-B, protein.
Lane 4 : purified
recombinant SPANX-C, protein.
Lane 5 : purified
recombinant SPANX-N, protein.
Secondary
Lanes 1-2 : IRDye™800 conjugated Gt-a-
Rabbit IgG [H&L] MX (611-132-122)
Predicted band size: 11 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 70 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
