Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733)
![Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733)](https://www.abcam.com/ps/products/50/ab50733/Images/ab50733-62409-anti-eif2s1-antibody-eif2s1a2b8-western-blot.gif "Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733)")
Key features and details
- Mouse monoclonal [EIF2S1A2B8] to EIF2S1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-EIF2S1 antibody [EIF2S1A2B8]
See all EIF2S1 primary antibodies -
Description
Mouse monoclonal [EIF2S1A2B8] to EIF2S1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant Human EIF2S1
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Positive control
- HEK293 and NIH3T3 whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.1136% Dibasic monohydrogen sodium phosphate, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab50733 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
EIF2S1A2B8 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733)Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733) at 1/50 dilution + HEK293 whole cell lysate at 50 µg
Secondary
Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry/ Immunofluorescence - Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733)](https://www.abcam.com/ps/products/50/ab50733/Images/ab50733-62408-anti-eif2s1-antibody-eif2s1a2b8-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733)ab50733 at 1/1 dilution staining human EIF2S1 in HeLa Cells by Immunocytochemistry. A 1/200 dilution of Alexa Fluor 488 anti-mouse IgG is used as a secondary. -
![Western blot - Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733)](https://www.abcam.com/ps/products/50/ab50733/Images/ab50733-62407-anti-eif2s1-antibody-eif2s1a2b8-western-blot.jpg)
Western blot - Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733)Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
anti-Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
