Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Thursday March 04, 2021

Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4082] to TCP1 alpha/CCTA
Suitable for: WB, IP, IHC-P
Reacts with: Human

Product name

Anti-TCP1 alpha/CCTA antibody [EPR4082]
See all TCP1 alpha/CCTA primary antibodies
Description

Rabbit monoclonal [EPR4082] to TCP1 alpha/CCTA
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, ECV-304, MCF7 and Daudi cell lysates; Human testis tissue
General notes
This product was previously labelled as TCP1 alpha

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4082
Isotype

IgG
Research areas

Western blot - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)

All lanes : Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126) at 1/1000 dilution

Lane 1 : HeLa cell lysates
Lane 2 : ECV-304 cell lysates
Lane 3 : MCF7 cell lysates
Lane 4 : Daudi cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 60 kDa
Immunoprecipitation - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)

ab109126 at 1:50 dilution (5μg in 1mg lysate) immunoprecipitating TCP1 alpha/CCTA in ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate.

Lane 1: ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate

Lane 2: PBS instead of ab109126 in ECV-304 whole cell lysate

For western blotting, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody at 1:1500 dilution.

Blocking and diluting buffer: 5% NFDM/TBST.

All lanes : Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126) at 1/1000 dilution

Lane 1 : ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate
Lane 2 : PBS instead of ab109126 in ECV-304 whole cell lysate

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

Observed band size: 60 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)

ab109126, at 1/100 dilution staining TCP1 in paraffin-embedded Human testis tissue, by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com