Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4082] to TCP1 alpha/CCTA
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-TCP1 alpha/CCTA antibody [EPR4082]
    See all TCP1 alpha/CCTA primary antibodies

  • Description

    Rabbit monoclonal [EPR4082] to TCP1 alpha/CCTA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, ECV-304, MCF7 and Daudi cell lysates; Human testis tissue

  • General notes

     

     

     This product was previously labelled as TCP1 alpha

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)
    Western blot - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)
    All lanes : Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : ECV-304 cell lysates
    Lane 3 : MCF7 cell lysates
    Lane 4 : Daudi cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 60 kDa

  • Immunoprecipitation - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)
    Immunoprecipitation - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)

    ab109126 at 1:50 dilution (5μg in 1mg lysate) immunoprecipitating TCP1 alpha/CCTA in ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate. 

     

    Lane 1: ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate

    Lane 2: PBS instead of ab109126 in ECV-304 whole cell lysate


    For western blotting, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody at 1:1500 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.



    All lanes : Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126) at 1/1000 dilution

    Lane 1 : ECV-304 (human urinary bladder cancer epithelial cell) whole cell lysate
    Lane 2 : PBS instead of ab109126 in ECV-304 whole cell lysate

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)

    ab109126, at 1/100 dilution staining TCP1 in paraffin-embedded Human testis tissue, by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)
    Anti-TCP1 alpha/CCTA antibody [EPR4082] (ab109126)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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