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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin

Anti-alpha smooth muscle Actin antibody [SPM332] (ab270251)

Anti-alpha smooth muscle Actin antibody [SPM332] (ab270251)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [SPM332] to alpha smooth muscle Actin
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-alpha smooth muscle Actin antibody [SPM332]
    See all alpha smooth muscle Actin primary antibodies
  • Description

    Mouse monoclonal [SPM332] to alpha smooth muscle Actin
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Rabbit, Goat, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Monkey, Baboon
  • Immunogen

    Synthetic peptide within Human alpha smooth muscle Actin conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
    Database link: P62736

  • Positive control

    • IHC-P: Human leiomyosarcoma tissue. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from Bioreactor Concentrate.
  • Clonality

    Monoclonal
  • Clone number

    SPM332
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins
    • Cardiovascular
    • Vasculature
    • Vasculature Markers
    • Arterial
    • Cardiovascular
    • Vasculature
    • Smooth muscle cell (SMC)
    • Cytoskeleton
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Smooth Muscle Cells

Images

  • Immunofluorescence - Anti-alpha smooth muscle Actin antibody [SPM332] (ab270251)
    Immunofluorescence - Anti-alpha smooth muscle Actin antibody [SPM332] (ab270251)

    Immunofluorescence analysis of HeLa cells labeling alpha smooth muscle Actin using ab270251 at 2µg/ml, followed by goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot (Red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [SPM332] (ab270251)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [SPM332] (ab270251)

    Formalin-fixed, paraffin-embedded human leiomyosarcoma tissue stained for alpha smooth muscle Actin using ab270251 at 2µg/ml in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: Flow Cyt, ICC/IF, IHC-P, WB

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    Applications: ICC/IF

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