Anti-SIRT3 antibody (ab189860)
Key features and details
- Rabbit polyclonal to SIRT3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SIRT3 antibody
See all SIRT3 primary antibodies -
Description
Rabbit polyclonal to SIRT3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human SIRT3 aa 1 to the C-terminus.
Database link: Q9NTG7 -
Positive control
- WB: HeLa cell extracts. IHC: human thyroid cancer, rat kidney, mouse kidney.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SIRT3 antibody (ab189860) at 1/500 dilution
Lane 1 : HeLa cells extracts
Lane 2 : SIRT3 knockout (KO) HeLa cells extracts
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 3 minutes
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Paraffin-embedded human thyroid cancer stained for SIRT3 using ab189860 at 1:100 dilution in immunohistochemical analysis.
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Anti-SIRT3 antibody (ab189860) at 1/1000 dilution + Extracts of SW620 cells at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 180 seconds
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Paraffin-embedded rat kidney tissue stained for SIRT3 using ab189860 at 1:100 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse kidney tissue stained for SIRT3 using ab189860 at 1:100 dilution in immunohistochemical analysis.