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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation

Anti-SIRT3 antibody (ab189860)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-SIRT3 antibody (ab189860)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to SIRT3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SIRT3 antibody
    See all SIRT3 primary antibodies
  • Description

    Rabbit polyclonal to SIRT3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human SIRT3 aa 1 to the C-terminus.
    Database link: Q9NTG7

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HeLa cell extracts. IHC: human thyroid cancer, rat kidney, mouse kidney.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class III / Sir2 class
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Metabolism
    • Types of disease
    • Obesity

Images

  • Western blot - Anti-SIRT3 antibody (ab189860)
    Western blot - Anti-SIRT3 antibody (ab189860)
    All lanes : Anti-SIRT3 antibody (ab189860) at 1/500 dilution

    Lane 1 : HeLa cells extracts
    Lane 2 : SIRT3 knockout (KO) HeLa cells extracts

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP Goat Anti-Rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 44 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody (ab189860)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody (ab189860)

    Paraffin-embedded human thyroid cancer stained for SIRT3 using ab189860 at 1:100 dilution in immunohistochemical analysis. 

  • Western blot - Anti-SIRT3 antibody (ab189860)
    Western blot - Anti-SIRT3 antibody (ab189860)
    Anti-SIRT3 antibody (ab189860) at 1/1000 dilution + Extracts of SW620 cells at 25 µg

    Secondary
    HRP Goat Anti-Rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 44 kDa


    Exposure time: 180 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody (ab189860)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody (ab189860)

    Paraffin-embedded rat kidney tissue stained for SIRT3 using ab189860 at 1:100 dilution in immunohistochemical analysis. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody (ab189860)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody (ab189860)

    Paraffin-embedded mouse kidney tissue stained for SIRT3 using ab189860 at 1:100 dilution in immunohistochemical analysis. 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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